A) WT or cct4-1 cells carrying Pab1-GFP imaged after shifting to 37°C (60min) either before NaN3 stress, after NaN3 treatment for 10min, after NaN3 treatment for 20min or with 1M KCl for 30min. (B) WT or cct4-1 cells carrying Pab1-GFP imaged prior to stress, after 30min of NaN3, after 60min of recovery at 37°C. Graph shows mean from 3 experiments ± standard deviation. (C) WT and mcm2-1 yeast cells with Pab1 GFP prior to stress, after stress (30min), and upon recovery from stress. Graph shows mean from 3 experiments ± standard deviation. (D) Same as (C) but for rvb2-1 and the corresponding WT yeast cells. * p-value < 0.05. ** p-value < 0.01. *** p-value < 0.0001. White scale bars indicate 2μm. Jain S, Wheeler JR, Walters RW, Agrawal A, Barsic A, Parker R. ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure. Cell. 2016 Jan 28;164(3):487-98. doi: 10.1016/j.cell.2015.12.038. Epub 2016