Knockdown of PTEX proteins EXP2 and PTEX150 reduces protein export into host erythrocytes. Export of Maurer’s cleft SBP1 protein after one cycle of GlcN treatment at 8 hpi rings by widefield microscopy in (A) 3D7, (B) EXP2-HAglmS and (C) PTEX150-HAglmS parasites. (Top) Quantitation of the number of SBP1 puncta in the parasites from n = number of individual cells counted. Kruskall-Wallis test used **p<0.005, *** p<0.01, p <0.0001. (Bottom) Representative immunofluorescence images showing reduced export of SBP1 structures after treatment of parasites with 0, 0.5 and 2 mM GlcN. Parasites were probed with rabbit anti-SBP1 serum (green), EXP2 mouse monoclonal IgG (red) and merged with DAPIDNA stain(blue). BF, brightfield. The brightness and contrast for SBP1 and EXP2 were equally adjusted for each image based on 3D7, 0 mMGlcN. (D) Images of GlcN-treated EXP2-H AglmS and PTEX150-HAglmS parasites in which the SBP1 and EXP2 signal has been enhanced. All size bars = 5 μm. (E) Diagram showing how knockdown of EXP2blocks protein export. RBC, red blood cell; PV, parasitophorous vacuole; Nu, nucleus; MC, Maurer’s cleft. Charnaud SC, Kumarasingha R, Bullen HE, Crabb BS, Gilson PR. Knockdown of the translocon protein EXP2 in Plasmodium falciparum reduces growth and protein export. PLoS One. 2018 13(11):e0204785. PMID: 30439948;