Expression and subcellular localization of PfRieske in P. falciparum. (A,B). Immunofluorescence assay of PfRieske, visualized with anti-HA, in asexual blood stages (A) and in gametocytes (B). (C,D) Immunofluorescence assay of PfRieske visualized with anti-HA and co-stained with MitoTracker-Deep-Red showing colocalization in both asexual (C) and sexual (D) blood stage parasites. Scale bar: 5 µm. (E) SDS-PAGE and western blot analysis to detect PfRieske-HA across the different developmental stages of the asexual blood stage cycle. Aldolase was used as a loading control. Sheokand PK, Pradhan S, Maclean AE, Mühleip A, Sheiner L. Plasmodium falciparum Mitochondrial Complex III, the Target of Atovaquone, Is Essential for Progression to the Transmissible Sexual Stages. Int J Mol Sci. 2024 25(17):9239. PMID: 39273187