Cellular localisation of invasins in P. falciparum schizonts . IFA of schizonts from HA tagged P. falciparum parasite lines labeled with anti-PfRON4, to mark the rhoptry neck, DAPI, to mark nuclei and anti-HA. (A) PfRON2 (B) PFF0645c (C) PfASP and (E) PF14_0578. (D) PF14_0375 was also colabeled with MitoTrackerH Deep Red, labeling mitochondria, and anti-PfACP, labeling apicoplasts. Scale bar=5 mm throughout. Zuccala ES, Gout AM, Dekiwadia C, Marapana DS, Angrisano F, Turnbull L, Riglar DT, Rogers KL, Whitchurch CB, Ralph SA, Speed TP, Baum J. Subcompartmentalisation of proteins in the rhoptries correlates with ordered events of erythrocyte invasion by the blood stage malaria parasite. PLoS One. 2012 7(9):e46160.