. coli DPCK complementation in the apicoplast and the deletion of DPCK. B Live epifluorescence microscopy of PfMev parasites that are expressing api-SFG (green) and api-EcDPCK-mCherry (red) with DAPI marking the nuclei (blue). The PfMev parasite line also expresses the green fluorescent protein, super-folder green (SFG), with the N-terminal trafficking peptide of the P. falciparum acyl-carrier protein (api-SFG), which directs its trafficking to the apicoplast, thereby fluorescently labeling the organelle. D Live epifluorescence microscopy of the PfMev api-EcDPCK-mCherry Ddpck parasite line that is expressing api-SFG (green) and api-EcDPCK-mCherry (red) with DAPI marking the nuclei (blue). E Live epifluorescence microscopy of the PfMev api-EcDPCK-mCherry Ddpck line after treatment with 1x azithromycin for 7 days under supplementation with 50 mM mevalonate. This parasite line expresses api-SFG (green) and api-EcDPCK-mCherry (red) with DAPI marking the nuclei (blue).

Swift RP, Rajaram K, Liu HB, Prigge ST. Dephospho-CoA kinase, a nuclear-encoded apicoplast protein, remains active and essential after Plasmodium falciparum apicoplast disruption. EMBO J. 2021 40(16):e107247.