Expression of ISP1 and ISP3 during parasite development. A) Expression of ISP1-GFP in schizont (asex), activated gametocytes (male and female), zygote,

ookinete (ook), and mid-gut (gut spz) and salivary gland (gland spz) sporozoites. Scale bar 5 5 mm. B) Expression of ISP3-GFP in segmenting schizont (asex), merozoites (mero), activated female gametocyte, zygote, ookinete (ook), and mid-gut (gut spz) and salivary gland (gland spz) sporozoites. For A) and B) a Cy3-conjugated antibody recognizing P28 on the surface of activated females, zygotes and ookinetes was used, nuclei were detected using Hoechst dye, and the cells were displayed by differential interference contrast (DIC). Merge is the composite of Hoechst, GFP and P28. Scale bar 5 5 mm. Expression of ISP1-GFP (C) and ISP3-GFP (D) in hepatocytic cells in vitro at 5 h and 48 h post invasion. An antibody to HSP70 detected parasites, hepatocyte nuclei were stained with Hoechst and the cells were displayed by differential interference contrast (DIC). Merge is the composite of Hoechst, GFP and HSP70. Scale bar 5 5 mm. E) Expression of ISP1-GFP and ISP3-GFP in liver merozoites (liver mero). Panels as in C and D. Scale bar 5 5 mm. Poulin B, Patzewitz EM, Brady D, Silvie O, Wright MH, Ferguson DJ, Wall RJ, Whipple S, Guttery DS, Tate EW, Wickstead B, Holder AA, Tewari R. Unique apicomplexan IMC sub-compartment proteins are early markers for apical polarity in the malaria parasite. Biol Open. 2013 2(11):1160-70. PMID: 24244852