Mutational analysis of the minimal sequence requirements for IMC association of PfISP3. AB.

Expression and localization of the 20 amino acid N-terminus with the last 10 (20PfISP311-20A-GFP) amino acids substituted with alanines. A1. 20PfISP311-20A-GFP was localized in unfixed parasites and showed an identical dynamic as the wild type protein.. B. The first 10 amino acids of PFISP3 (10PfISP3-GFP) are sufficient for IMC localization (B1) B2. Mutation of the sole charged residue D10 in to a neutral glycine (10PfISP3D10G-GFP, C1) or a positively charged arginine

(10PfISP3D10R-GFP, D1) does not interfere with IMC targeting.

Wetzel J, Herrmann S, Swapna LS, Prusty D, Peter AT, Kono M, Saini S, Nellimarla S, Wong TW, Wilcke L, Ramsay O, Cabrera A, Biller L, Heincke D, Mossman K, Spielmann T, Ungermann C, Parkinson J, Gilberger TW. The role of palmitoylation for protein recruitment to the inner membrane complex of the malaria parasite. J Biol Chem. 2014 Nov 25.