The role of N-terminal acylation for IMC membrane association of PfISP1 and acyl biotin exchange assays. A. Over-expression of PfISP1-GFP showing the same characteristic IMC dynamics as PfISP3-GFP. Putative myristoylation and palmitoylation sites are highlighted in light blue (G2) or green (C7C8). B-C. Mutation of either one of the acylation motifs (20PfISP1G2A-GFP and 20PfISP1C7AC8A-GFP) resulted in a cytosolic variant shown by microscopy. Nuclei are stained with DAPI (blue). Enlargement of selected areas are marked with a white square and referred to as zoom. Scale bar, 1 μm.

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