N Termini of PNEPs Are Sufficient for Export of RREX2-TM (A) Schematic of mTRAP fusion constructs. (B) Representative images of live P. falciparum parasites expressing the constructs shown in (A). DIC, differential interference contrast; nuclei were stained with DAPI. Arrows indicate limited staining reminiscent of Maurer’s clefts. (C and D) Images of live P. falciparum parasites expressing RREX2-TM (C) and RSBP1-TM (D) fused with the PNEP N termini indicated. Panels are as in (B). Size bars represent 5 mm. The mTRAP backbone, different TMs, and the SP (signal peptide) are shown in different shades of gray; N-terminally appended regions of PNEPs are shown in shades from yellow to dark red. Ce-TM, C .elegans TKTM. See also Figure S. Grüring C, Heiber A, Kruse F, Flemming S, Franci G, Colombo SF, Fasana E, Schoeler H, Borgese N, Stunnenberg HG, Przyborski JM, Gilberger TW, Spielmann T. Uncovering common principles in protein export of malaria parasites. Cell Host Microbe. 2012 12(5):717-29. PMID: 23159060.