Live cell time course showing assembly of exomembrane components. Transfectants expressing GFP chimeras of (A, B) REX1 and (C, D) MAHRP2 were co-labeled with BODIPYceramide. Infected RBCs were synchronized to a ~2 h window and samples were collected at 4 h intervals. BODIPY-ceramide- and REX1-GFP-labeled Maurer's clefts and MAHRP2-GFP-labled structures are evident in the RBC cytoplasm from ~4 h post invasion. MAHRP2-GFP-labled structures are associated with the BODIPY-ceramide-labeled Maurer's clefts (D, white arrows). (F) MAHRP2-GFP transfectants were permeabilized with EqtII and labeled with antibodies recognizing REX1. Scale bars = 3 μm (A, C), 1 μm (B, D) and 4 μm (F). McMillan PJ, Millet C, Batinovic S, Maiorca M, Hanssen E, Kenny S, Muhle RA, Melcher M, Fidock DA, Smith JD, Dixon MW, Tilley L. Spatial and temporal mapping of the PfEMP1 export pathway in Plasmodium falciparum. Cell Microbiol. 2013 15(8):1401-18.