Dual label immunofluorescence microscopy of formaldehyde or acetone/methanol-fixed 3D7 smears using antibodies recognizing MAHRP2 (green) and anti-MAHRP1, anti-SBP1 and anti-PfEMP1 (red), or (last panel) anti-MAHRP1 (green) and anti-SBP1 (red). Scale bar = 5 mm. In mature stage parasites the antibodies reacted with punctate structures in the erythrocyte cytoplasm in a pattern that is somewhat reminiscent of Maurer’s cleft staining. No colocalization of MAHRP2 could be found with MAHRP1 MAL13P1.413, SBP1 PFE0065w or PfEMP1; however, the signals were often in close proximity. This suggests that MAHRP2 is associated with a separate compartment of the exomembrane system of P. falciparum.

Pachlatko E, Rusch S, Müller A, Hemphill A, Tilley L, Hanssen E, Beck HP. MAHRP2, an exported protein of Plasmodium falciparum, is an essential component of Maurer's cleft tethers. Mol Microbiol. 2010 77(5):1136-52