Representative IFA images confirming loss of MSA180-HA3 expression following RAP treatment (reproducible in 2 independent experiments). Mature C2-arrested cycle 0 schizonts were co-stained with anti-HA (red) and anti-SERA5 (green). Merged signals include that of the DNA dye 4,6-diamidino-2-phenylindole (DAPI; blue). Scale bar, 5 mm. Partial co-localisation of the IFA signal with SERA5, a soluble PV protein, suggested localization of MSA180 to the PV.

Tan MSY, Koussis K, Withers-Martinez C, Howell SA, Thomas JA, Hackett F, Knuepfer E, Shen M, Hall MD, Snijders AP, Blackman MJ. Autocatalytic activation of a malarial egress protease is druggable and requires a protein cofactor. EMBO J. 2021 1:e107226.