Plasma membrane association is lost in parasites expressing the truncated PfPLSCR-GFP fusion protein, which distributes throughout the parasite cytosol. Schematic of the CRISPR-Cas9 mediated KO strategy. Expression of sfGFP is driven under a constitutive BiP promoter. Live-cell image of PfPLSCR KO parasites expressing sfGFP throughout asexual parasite development. Parasite nuclei are stained with DAPI (blue).
Haase S, Condron M, Miller D, Cherkaoui D, Jordan S, Gulbis JM, Baum J. Identification and characterisation of a phospholipid scramblase in the malaria parasite Plasmodium falciparum. Mol Biochem Parasitol. 2021 8:111374.
PubMed Article: Identification and characterisation of a phospholipid scramblase in the malaria parasite Plasmodium falciparum
Other associated proteins
|PF3D7_1022700||phospholipid scramblase, plscr|