Depletion of PfAP2-HC has no marked effect on re-establishment of heterochromatin. Representative live cell fluorescence images showing PfHP1-mScarlet localization in stage II gametocytes (C) and stage V gametocytes (D) grown under DDGFP-PfAP2-HC-stabilizing (+Shield-1, upper two panels) and -depleting (Shield-1, lower two panels) conditions. Nuclei were stained with Hoechst. DIC, differential interference contrast. Scale bar, 5 mm. We observed no marked difference in the localization pattern of PfHP1-mScarlet between gametocytes that express or do not express DDGFP-PfAP2-HC. These observations indicate that PfAP2-HC likely plays no major role in de novo heterochromatin formation.
Carrington E, Cooijmans RHM, Keller D, Toenhake CG, Bártfai R, Voss TS. The ApiAP2 factor PfAP2-HC is an integral component of heterochromatin in the malaria parasite Plasmodium falciparum. iScience. 2021 24(5):102444
Other associated proteins
|PF3D7_1456000||AP2 domain transcription factorAP2-HC|