Upper panel: Representative IFA images of GFP-PfAP2-HC-DDBD and PfHP1 localization in a developing schizont (36–44 hpi). Nuclei were stained with DAPI. DIC, differential interference contrast. Scale bar, 5 mm. The localization of GFP-PfAP2-HCDDBD is comparable with that of GFP-PfAP2-HC by IFA and similarly shares this localization pattern with PfHP1. Lower panel: Representative live cell fluorescence images of 3D7/GFP-PfAP2-HC/PfHP1-mScarlet-glmS parasites at 32–40 hpi grown in the absence of GlcN (PfHP1 expressed) or the presence of GlcN (PfHP1 depleted). Nuclei were stained with Hoechst. DIC, differential interference contrast. Scale bar, 5 mm

Carrington E, Cooijmans RHM, Keller D, Toenhake CG, Bártfai R, Voss TS. The ApiAP2 factor PfAP2-HC is an integral component of heterochromatin in the malaria parasite Plasmodium falciparum. iScience. 2021 24(5):102444