Coexpression of PICs with the IMC marker ALV5-mCherry. Coexpression of the candidate proteins PIC1 (a), PIC2 (b), PIC3 (c), PIC4 (d) and PIC6 (e) with the IMC marker protein ALV5 episomally expressed as mCherry fusion protein under the control of the late-stage promoter ama1. (f) PIC5 episomally expressed as mCherry fusion protein under the control of the late-stage promoter ama1 in PhIL1-BirA-GFP parasites. Nuclei stained with Hoechst-33,342. Zoom factor: 400%. Scale bars 2 μm

Wichers JS, Wunderlich J, Heincke D, Pazicky S, Strauss J, Schmitt M, Kimmel J, Wilcke L, Scharf S, von Thien H, Burda PC, Spielmann T, Löw C, Filarsky M, Bachmann A, Gilberger TW. Identification of novel inner membrane complex and apical annuli proteins of the malaria parasite Plasmodium falciparum. Cell Microbiol. 2021 8:e13341.