Left: Representative images from immunofluorescence assays showing PfRAP1 localization in WT or CD55-null RBCs with attached merozoites in the presence of cyt-D. Assays were performed twice and ~50 attached merozoites were assessed for each RBC genetic background. When free merozoites were allowed to attach to RBCs in the presence of cyt-D, we observed RAP1 staining in a donut or C-shape for merozoites attached to both the WT and CD55-null cells, indicating that rhoptry discharge had occurred. Right: Representative confocal images showing merozoites attached to RBCs with apical or non-apical orientation. Merozoite density at the apical end of the merozoite is indicated by an arrow. Using confocal microscopy, we found that PfAMA1 and PfRON4 were frequently co localized at the cellular interface for merozoites attached to WT control cells (~85%). In comparison, for merozoites attached to CD55-null erythrocytes, PfAMA1 and PfRON4 were co-localized at the cellular interface in only ~40% of cases. For those merozoites in which PfAMA1 and PfRON4 were colocalized at the interface, we observed that approximately half of the WT control cells had an indentation at the point of merozoite contact, suggesting the merozoites were mid invasion, whereas this was never observed for the CD55-null cells.
Shakya B, Patel SD, Tani Y, Egan ES. Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites. Elife. 2021 May 10:e61516.
Other associated proteins
|PF3D7_1116000||rhoptry neck protein 4|
|PF3D7_1410400||rhoptry-associated protein 1|