PfRhopH3 protein is a ligand for Cyclophilin B protein during merozoite binding. a Binding of Cyclophilin B on Merozoite surface. Binding shows binding of CypB on merozoite surface without treatment with any antibody. anti-Rap2b + CypB and anti-RhopH3-C + CypB show binding after treatment with anti-Rap2b and anti-RhopH3 antibody respectively. Intensity for each merozoite is mentioned at the top. Images with histograms represent only the intensity of the bound CypB protein. Anti-RhopH3-C antibody interferes with binding of CypB on merozoites, signifying that PfRhopH3 is a ligand for host Cyclophilin B. Scale bar = 5 μm. More than 50% reduction in Cyclophilin B binding to merozoite surface was observed when the merozoite surface was treated with anti-RhopH3-C antibody, in comparison to merozoites treated with anti-RAP2 antibody or untreated cells.

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