Left: ABCI3 foci localize to punctate structures in the parasite cytosol and occasionally with the nucleus, ER, or DV ABCI3 FLAG-tagged parasites were stained with DAPI (40,6-diamidino-2-phenylindole) (nucleus, blue) and antibodies specific to FLAG (green), PfCRT (DV membrane, red), and BiP (ER, magenta). Scale bars, 2 mm. No substantial colocalization was observed with the digestive vacuole (DV) marker PfCRT, the ER marker binding immunoglobulin protein (BiP). Right: IEM image of an HA-tagged ABCI3 trophozoite stained with anti-HA antibodies, revealing staining in the cytosol, nucleus, nuclear membrane, and ER. Arrowheads highlight organelles of interest. C, cytosol; ER, endoplasmic reticulum; N, nucleus; NM, nuclear membrane; Hz, hemozoin crystals. Scale bar, 500 nm. IEM analysis of at least eight parasites cultured independently in triplicate (31 total images) localized ABCI3 49% of the time to the cytosol and 24% to the nucleus and nuclear membrane. Other sites of localization included the DV (8%), the plasma membrane (6%), and the ER and intracellular vesicles (13%).
Murithi JM, Deni I, Pasaje CFA, Okombo J, Bridgford JL, Gnädig NF, Edwards RL, Yeo T, Mok S, Burkhard AY, Coburn-Flynn O, Istvan ES, Sakata-Kato T, Gomez-Lorenzo MG, Cowell AN, Wicht KJ, Le Manach C, Kalantarov GF, Dey S, Duffey M, Laleu B, Lukens AK, Ottilie S, Vanaerschot M, Trakht IN, Gamo FJ, Wirth DF, Goldberg DE, Odom John AR, Chibale K, Winzeler EA, Niles JC, Fidock DA. The Plasmodium falciparum ABC transporter ABCI3 confers parasite strain-dependent pleiotropic antimalarial drug resistance. Cell Chem Biol. 2021 5:S2451-9456(21)00305-6.
Other associated proteins
|PF3D7_0319700||ABC transporter I family member 1, putative, ABCI3|
|PF3D7_0709000||chloroquine resistance transporter|