Downregulation of P. falciparum apicortin trans-expressed in HEK 293T cells by miR-150 and miR-197. Immunofluorescence assay images of transfected cells with PfApicortin in the green channel and tubulin (as control) in the red channel; individual cells are shown in magnified form. PfApicortin expression was reduced in cells transfected with miR-150 (P<0.001) or miR-197. Nuclei were counterstained with DAPI. Scale bars: 5 μm. Immunofluorescence assays showed a significant reduction of fluorescence intensity of PfApicortin in miRNA-cotransfected cells compared with controls.
Chakrabarti M, Joshi N, Kumari G, Singh P, Shoaib R, Munjal A, Kumar V, Behl A, Abid M, Garg S, Gupta S, Singh S. Interaction of Plasmodium falciparum apicortin with α- and β-tubulin is critical for parasite growth and survival. Sci Rep. 2021 11(1):4688.
Other associated proteins
|PF3D7_0422300||alpha tubulin 2, transcript expression profile microgametocyte|