Localization of PfLPL1 by immune-electron microscopy. Ultra-thin sections of transgenic P. falciparum parasites expressing PfLPL1-GFPDDD

were labelled with anti-GFP antibody and gold labelled secondary antibody: a in young parasites, labelling was observed around nucleus in the endoplasmic reticulum; b in some young parasite, labelling was in the putative ER exit site; c in trophozoite stages, labelling was observed in small vesicles (< 100 nm) in the parasite cytosol; d, e in mature parasites, labelling was observed in large vesicular structure (> 200 nm) localized in close association with the food-vacuole (having characteristic hemozoin). Scale bar = 200 nm. f PfLPL1 associate with neutral lipid storage body near food vacuole. Fluorescence images of trophozoites stage transgenic parasites expressing PfLPL1-GFP-DDD stained with Nile Red, a neutral

lipid staining dye. The large GFP-labelled structure close to the food vacuole (having dark hemozoin) showed staining with Nile Red. The parasite nuclei were stained with DAPI (blue) and parasites were visualized by confocal laser scanning microscope

Asad M, Yamaryo-Botté Y, Hossain ME, Thakur V, Jain S, Datta G, Botté CY, Mohmmed A. An essential vesicular-trafficking phospholipase mediates neutral lipid synthesis and contributes to hemozoin formation in Plasmodium falciparum. BMC Biol. 2021 19(1):159.