Expression and localization of the PfLPLl fusion protein with ddFKBP degradation domain (PfLPLl-DD) in transgenic parasites. (A) Fluorescence microscopy images of PfLPLl-DD transgenic parasite immuno-stained with anti-HA antibody. (B) Fluorescence microscopy images of PfLPLl-DD transgenic parasite immuno-stained with anti-HA antibody and co-stained with Nile red . The parasite nuclei were stained with DAPI and slides were visualized by confocal microscope. The PfLPL1 was localized in vesicular structures which overlapped in neutral lipid body in late trophozoite stages.

Asad M, Yamaryo-Botté Y, Hossain ME, Thakur V, Jain S, Datta G, Botté CY, Mohmmed A. An essential vesicular-trafficking phospholipase mediates neutral lipid synthesis and contributes to hemozoin formation in Plasmodium falciparum. BMC Biol. 2021 19(1):159.