Generation of transgenic parasite expressing RFA-tagged (DDD-GFP) PfLPL1 and localisation of PfLPL1-GFP-DDD fusion protein in transgenic parasites. Localization of PfLPL1-GFPDDD fusion protein in transgenic P. falciparum parasite cultures synchronized and visualized at different developmental stages. Fluorescent microscopic images of live transgenic parasites at ring (16–18 hpi), trophozoite (30 hpi), and schizont (42 hpi) stages. The parasite nuclei were stained with DAPI; fluorescence as well as DIC (differential interference contrast) images were obtained by confocal laser scanning microscope. NIS element software (version 4.0) was used to merge images obtained from different fluorescence/DIC images to produce the merged images. In ring stages, the GFP-fluorescence was observed around the nucleus; in early trophozoite stages, small vesicles are present near the parasitophorous vacuole and in the parasite cytosol. In mature stages, late trophozoite stage, and schizonts, GFP-fluorescence was observed in large vesicular structure in close association with the food-vacuole.
Asad M, Yamaryo-Botté Y, Hossain ME, Thakur V, Jain S, Datta G, Botté CY, Mohmmed A. An essential vesicular-trafficking phospholipase mediates neutral lipid synthesis and contributes to hemozoin formation in Plasmodium falciparum. BMC Biol. 2021 19(1):159. PMID: 34380472.