Live-cell fluorescence assays of PfAP2-G signal (green) with late stage parasites (30–40 hpi) from 3D7, pfap2-g5 trun, pfap2-g5 dDBD lines carrying PfAP2-G-GFP fusion protein. The nuclei are stained with DAPI (blue). Scale bar: 5 mm. three ApiAP2 genes [PF3D7 1342900, PF3D7 1317200 (putative AP2-G3), PF3D7 1222400] positively correlated with pfap2-g transcription and gametocytogenesis, i.e. the knockout lines exhibited significantly lower levels of pfap2-g transcripts as well as reduced gametocyte production.

Shang X, Shen S, Tang J, He X, Zhao Y, Wang C, He X, Guo G, Liu M, Wang L, Zhu Q, Yang G, Jiang C, Zhang M, Yu X, Han J, Culleton R, Jiang L, Cao J, Gu L, Zhang Q. A cascade of transcriptional repression determines sexual commitment and development in Plasmodium falciparum. Nucleic Acids Res. 2021 gkab683