Electron microscopy reveals deformed knob morphologies of ΔPFA iRBCsD) Internal view of the deformed knobs/eKnob via transmission electron microscopy of thin slices. Electron tomography reveals electron-dense material at the base and interior of deformed knobs/eKnobs. The marked area denotes the structure shown in F. F) 3D segmentation of discrete densities within a deformed knob/eKnob depicted with electron tomography. The example shows a severely deformed knob/eKnob. Additionally, electron dense material was detected at the base and inside of
Diehl M, Roling L, Rohland L, Weber S, Cyrklaff M, Sanchez CP, Beretta CA, Simon CS, Guizetti J, Hahn J, Schulz N, Mayer MP, Przyborski JM. Co-chaperone involvement in knob biogenesis implicates host-derived chaperones in malaria virulence. PLoS Pathog. 2021 Oct 6;17(10):e1009969. doi: 10.1371/journal.ppat.1009969. Epub ahead of print.