PF3D7_0414900 armadillo-domain containing rhoptry protein

IMP-1002 treatment during schizogony has no effect on formation of the IMC but affects the subcellular location of ARO and RON4 and rhoptry biogenesis. Light microscopy images from indirect IFAs performed in duplicate on 3 separate occasions with fixed parasites from DMSO (control) and IMP-1002–treated parasites and protein-specific antibodies. Panels show the DIC image, the specific antibody location (green or red), and a merged image of antibody staining with DAPI staining of nuclei. Scale bar = 5 μm. (A) Two

examples showing that the location of GAP45 and MyoA at the IMC appeared largely unchanged following drug treatment. (B) Two examples showing that the location of ARO and RON4 was affected by drug treatment, with the proteins appearing to be distributed throughout the cytoplasm of developing merozoites and a loss of distinct rhoptry staining in treated parasites.

Schlott AC, Knuepfer E, Green JL, Hobson P, Borg AJ, Morales-Sanfrutos J, Perrin AJ, Maclachlan C, Collinson LM, Snijders AP, Tate EW, Holder AA. Inhibition of protein N-myristoylation blocks Plasmodium falciparum intraerythrocytic development, egress and invasion. PLoS Biol. 2021 19(10):e3001408.

Other associated proteins

PFID Formal Annotation
PF3D7_1116000 rhoptry neck protein 4
PF3D7_1222700 glideosome-associated protein 45
PF3D7_1342600 myosin A