IMP-1002 treatment during schizogony has no effect on formation of the IMC but affects the subcellular location of ARO and RON4 and rhoptry biogenesis. Light microscopy images from indirect IFAs performed in duplicate on 3 separate occasions with fixed parasites from DMSO (control) and IMP-1002–treated parasites and protein-specific antibodies. Panels show the DIC image, the specific antibody location (green or red), and a merged image of antibody staining with DAPI staining of nuclei. Scale bar = 5 μm. (A) Two
examples showing that the location of GAP45 and MyoA at the IMC appeared largely unchanged following drug treatment. (B) Two examples showing that the location of ARO and RON4 was affected by drug treatment, with the proteins appearing to be distributed throughout the cytoplasm of developing merozoites and a loss of distinct rhoptry staining in treated parasites.
Schlott AC, Knuepfer E, Green JL, Hobson P, Borg AJ, Morales-Sanfrutos J, Perrin AJ, Maclachlan C, Collinson LM, Snijders AP, Tate EW, Holder AA. Inhibition of protein N-myristoylation blocks Plasmodium falciparum intraerythrocytic development, egress and invasion. PLoS Biol. 2021 19(10):e3001408.
Other associated proteins
|PF3D7_1116000||rhoptry neck protein 4|
|PF3D7_1222700||glideosome-associated protein 45|