PfCSA-L co-localizes with VAR2CSA on the surface of CSA-binding iRBC. (A) IFA of live, intact iRBC of CSA-binding wild type and var2csa-disrupted parasites of 3D7 and CS2 cloned lines. Live iRBC were labeled with rabbit anti-PfCSA-L (red) or rat anti-DBL4-VAR2CSA (green). Images are representative of 3 independent assays. First and last panel represent phase contrast and overlay images respectively. Yellow color indicates co-localization; blue represents DAPI nuclei staining. (B) Visualization of the interaction between PfCSA-L and VAR2CSA on the surface of CSA-binding iRBC using Duolink assay. IRBC were stained with PfCSA-L mAb 1H11 and rabbit anti-VAR2CSA (top panels), with PfCSA-L mAb 1H11 and anti-mTRAP antibodies (bottom, left panel), with PyRON2 mAb and rabbit anti-VAR2CSA (bottom, middle panel), or with no primary antibody (bottom, right panel). Red spots indicate areas in which proteins are in close proximity. Cell nuclei are stained in blue with DAPI.
Keitany GJ, Jenkins BJ, Obiakor HT, Daniel S, Muehlenbachs A, Semblat JP, Gamain B, Doritchamou JYA, Desai SA, MacDonald NJ, Narum DL, Morrison R, Saveria T, Vignali M, Oleinikov AV, Fried M, Duffy PE. An invariant protein that co-localizes with VAR2CSA on Plasmodium falciparum-infected red cells binds to chondroitin sulfate A. J Infect Dis. 2021 Oct 29:jiab550PMID: 34718641.
Other associated proteins
|PF3D7_1001000||chondroitin sulfate a ligand; plasmodium exported protein (hyp12)|
|PF3D7_1028700||merozoite TRAP-like protein|