Mitochondrial localization of PfBlm and PfRad51. Immunofluorescence images displaying localization of PfBlm-GFP and PfRad51-GFP (green) to mitochondria (red) and/or nucleus (blue) of Plasmodium falciparum parasite. Anti-CytC antibody and Hoechst dye were used to stain parasite mitochondria and the nucleus, respectively. IFA using anti-GFP in 3D7 and preimmune sera in PfBlm-GFP transfected parasite served as the experimental negative controls. IFA was performed with trophozoite and schizont parasites as indicated. DIC, differential interference contrast. Colocalization of signals from GFP-tagged PfRad51 or PfBlm with CytC confirmed the mitochondrion localization of PfRad51 or PfBlm in both the trophozoite and schizont stages. As expected, GFP-tagged PfRad51 or PfBlm proteins were also found in the parasite nucleus.

Jha P, Gahlawat A, Bhattacharyya S, Dey S, Kumar KA, Bhattacharyya MK. Bloom Helicase Along with Recombinase Rad51 Repairs the Mitochondrial Genome of the Malaria Parasite. mSphere. 2021 e0071821.