Identification and characterization of a P. falciparum homolog of Get3. E. IFA images showing the expression and localization of PfGet3 (green) as diffused cytosolic fluorescence in ring (top panel), trophozoite (middle panel) and schizont (bottom panel) stages of infection. In trophozoites and schizonts, additional PfGet3-labeled structures as punctate spots or reticular pattens (white arrowheads) can be seen in the magnified versions of the dotted outlined regions. The parasite boundary is marked by antibodies to a parasitophorous membrane marker, PfExp-2 (red). Corresponding brightfield, single or merged fluorescent images with nuclear staining (blue) are shown. F. IFA images showing areas of colocalization between PfGet3 (green) and ER-resident proteins such as BiP (red, top panel) or Plasmepsin V (red, bottom panel) indicated by arrowheads in the magnified view of the dotted outlined regions. Corresponding brightfield, single or merged fluorescent images with nuclear staining (blue) are shown.
Kumar T, Maitra S, Rahman A, Bhattacharjee S. A conserved guided entry of tail-anchored pathway is involved in the trafficking of a subset of membrane proteins in Plasmodium falciparum. PLoS Pathog. 2021 Nov 15;17(11):e1009595.
Other associated proteins
|PF3D7_0415000||ATPase get3, putative, Get3|
|PF3D7_1471100||exported protein 2|