P. falciparum ApiATs localize to the parasite plasma membrane (PPM) during asexual blood stage development. Colocalization of the GFP-tagged ApiAT fusion proteins with the PPM marker protein Lyn-mCherry in schizonts and merozoites. (D) Live-cell microscopy of 3D7-crt-ApiAT10-GFP parasites across the IDC. colocalization with the episomally expressed PPM marker Lyn (39)-mCherry (38) reveals PPM localization that becomes particularly evident in free merozoites, where PPM- and PVM-localized proteins can clearly be separated.

Wichers JS, van Gelder C, Fuchs G, Ruge JM, Pietsch E, Ferreira JL, Safavi S, von Thien H, Burda PC, Mesén-Ramirez P, Spielmann T, Strauss J, Gilberger TW, Bachmann A. Characterization of Apicomplexan Amino Acid Transporters (ApiATs) in the Malaria Parasite Plasmodium falciparum. mSphere. 2021 6(6):e0074321.