PfCRMP2 is expressed on the sporozoite surface. A. Sporozoites emerging from oocysts in the mosquito midgut express PfCRMP2. Top panel: merged image of the fluorescence patterns of antisera detecting PfCRMP2 (red) on emerging sporozoites (arrowed) and DAPI (blue) detecting sporozoite nuclei within a mature and rupturing oocyst. The oocyst wall is impermeable to antisera and does not express PfCRMP2. Middle panel: PfCRMP2; bottom panel: DAPI. Scale bar = 10 mm. B and C. PfCRMP2 is localized in a punctate pattern over the surface of salivary gland sporozoites. Immunofluorescence assay was performed without permeabilization (B) or with permeabilization (C). Left hand panel: merged image of the fluorescence patterns of antisera detecting PfCRMP2 (red), AMA1 (green) and DAPI (blue). Middle panel: PfCRMP2. Right hand panel: AMA1. Surface-localized AMA1 released from sporozoite micronemes is arrowed in B. Scale bar = 5 mm. D. Salivary gland sporozoites express PfCRMP2. Merged image of the fluorescence patterns of antisera detecting PfCRMP2 (red) on bundles of sporozoites within a mosquito salivary gland and DAPI (blue) detecting the nuclei of sporozoites and mosquito cells (arrowed). Scale bar = 5 mm.
Kafatos FC, Janse CJ, Waters AP. Plasmodium cysteine repeat modular proteins 1-4: complex proteins with roles throughout the malaria parasite life cycle. Cell Microbiol. 2007 9(6):1466-80.
Other associated proteins
|PF3D7_0718300||cysteine repeat modular protein 2|