Assessment of overexpression of PfA-M1 in transgenic P. falciparum 3D7 parasite (over PfA-M1). (a) Green fluorescent protein (GFP) images of infected red blood cells overexpressing PfA-M1-GFP-HA (green). DNA was stained with DAPI (blue). A-M1 has a localization compatible with the cytosol, showing a homogeneous fluorescence distribution in the entire parasite cell and excluded from the hemozoin region.
Hoff CC, Azevedo MF, Thurler AB, Maluf SEC, Melo PMS, Del Rivero MA, González-Bacerio J, Carmona AK, Budu A, Gazarini ML. Overexpression of Plasmodium falciparum M1 Aminopeptidase Promotes an Increase in Intracellular Proteolysis and Modifies the Asexual Erythrocytic Cycle Development. Pathogens. 2021 10(11):1452.