Phosphorylation of EK S37 is important for parasite development. IFA was performed on parasites expressing WT EK-GFP or its S37A mutant 6–12 h post-invasion to detect (i) RAP1, which is transferred to PV post-invasion, and (ii)MSP1 which is located at the PVM. Parasite expressing WT EK-GFP exhibited typical round ring like shape. In contrast, several parasites expressing the S37A mutant exhibited irregular morphology with much smaller vacuolar space and in some parasites RAP1 was found outside the PV (Scale bar, 1 mm).
Maurya R, Tripathi A, Kumar M, Antil N, Yamaryo-Botté Y, Kumar P, Bansal P, Doerig C, Botté CY, Prasad TSK, Sharma P. PI4-kinase and PfCDPK7 signaling regulate phospholipid biosynthesis in Plasmodium falciparum. EMBO Rep. 2021 e54022.
Other associated proteins
|PF3D7_0930300||merozoite surface protein 1|
|PF3D7_1410400||rhoptry-associated protein 1|