A 3D7 parasites were treated with DMSO or 50 nM MMV048 for 24 h. Subsequently, parasites were fixed and IFA was performed to detect ER marker BiP and PfCDPK7. While PfCDPK7 was present in puncta proximal to ER (left panel), inhibitor treatment caused a loss in this vesicular localization and resulted in a more diffuse distribution of the enzyme (right panel) (Scale bar, 1 mm). The inhibition of PfPI4K impaired the localization of PfCDPK7 to these vesicular organelles. C. MMV048 treatment caused defects in PVM/PM development, as indicated by aberrant RAP1 localization. which was also observed in both PfCDPK7-KO.
Maurya R, Tripathi A, Kumar M, Antil N, Yamaryo-Botté Y, Kumar P, Bansal P, Doerig C, Botté CY, Prasad TSK, Sharma P. PI4-kinase and PfCDPK7 signaling regulate phospholipid biosynthesis in Plasmodium falciparum. EMBO Rep. 2021 e54022.
Other associated proteins
|PF3D7_0930300||merozoite surface protein 1|
|PF3D7_1123100||calcium-dependent protein kinase 7|