Targeted mutagenesis of PfPDK1 confirms its essential role in activating PfPKAc. (A, B) Expression of PfPKAc-GFP under OE-inducing (–GlcN) and control conditions (+GlcN) in NF54/PKAc cOE S1/PDK1_wt parasites (A) and NF54/PKAc cOE M1/PDK1_mut parasites (B) by live cell fluorescence imaging and western blot analysis. Synchronous parasites (0 to 8 hpi) were split (±GlcN) 40 hours before collection of the samples. Representative fluorescence images are shown. Parasite DNA was stained with Hoechst. Scale bar = 5 μm.

Hitz E, Wiedemar N, Passecker A, Graça BAS, Scheurer C, Wittlin S, Brancucci NMB, Vakonakis I, Mäser P, Voss TS. The 3-phosphoinositide-dependent protein kinase 1 is an essential upstream activator of protein kinase A in malaria parasites. PLoS Biol. 2021 Dec 8;19(12):e3001483.