Localisation and expression of APC3-GFP in mitotic cells. (A) Live imaging or (B to E) fixed IFA of APC3-GFP with centrin, α-tubulin (α-Tub) or γ-tubulin (γ-Tub) antibodies in schizonts, microgametocytes (gametocytes) and gametes. DIC: Differential interference contrast, Merge: GFP and Hoechst. Scale bar = 5 μm. Live cell imaging revealed cytoplasmic localisation of APC3-GFP in schizonts and microgametocytes (A). APC3-GFP was found to localise to specific foci in the cytoplasm. Co-staining with antibodies against the known centrosomal markers, centrin and ɣ-tubulin, revealed that in schizonts a single focus of APC3-GFP was present associated with each nucleus, which partially co-localised with a single focus of centrin and γ-tubulin; this is highly persuasive of an association of APC3 with the CPs in this stage of the life cycle (B). During male gametogenesis, multiple MTOC-like structures were observed with centrin and γ-tubulin antibodies. A few of these exhibited partial colocalisation with APC3-GFP foci but the majority did not ( C and D).
Wall RJ, Ferguson DJP, Freville A, Franke-Fayard B, Brady D, Zeeshan M, Bottrill AR, Wheatley S, Fry AM, Janse CJ, Yamano H, Holder AA, Guttery DS, Tewari R. Plasmodium APC3 mediates chromosome condensation and cytokinesis during atypical mitosis in male gametogenesis. Sci Rep. 2018 8(1):5610.
Other associated proteins
|PF3D7_0422300||alpha tubulin 2, transcript expression profile microgametocyte|
|PF3D7_0803700||tubulin gamma chain|