The PX domain of PfPX1 likely binds selectively to PI3P. (A) Representative images of Cos-7 cells co-transfected with EGFP-PfPXWT or EGFP-PfPXRKR and mCherry-tagged early endosome marker Rab5. Blue: Hoechst-stained DNA. Magenta: plasma membrane stained with wheat-germ hemagglutinin-Alexa fluor 647. White arrows show colocalization of EGFP signal with mCherry signal indicating PfPXWT binding to early endosomes. Zoom panel corresponds to the insets showed in Merge panel. (B) Representative confocal images of Cos-7 transfected with EGFP-PfPXWT treated with ethanol (EtOH) or 100 nM Wortmannin. EGFP fluorescence is more cytosolic and reduced at mCherry-Rab5 positive structures in Wortmannin-treated cells (i). (C) Representative confocal images of Cos-7 transfected with EGFP-PfPXWT treated with DMSO or 20 nM Apilimod (i).
Mukherjee A, Crochetière MÈ, Sergerie A, Amiar S, Thompson LA, Ebrahimzadeh Z, Gagnon D, Lauruol F, Bourgeois A, Galaup T, Roucheray S, Hallée S, Padmanabhan PK, Stahelin RV, Dacks JB, Richard D. A Phosphoinositide-Binding Protein Acts in the Trafficking Pathway of Hemoglobin in the Malaria Parasite Plasmodium falciparum. mBio. 2022 Jan 18;13(1):e0323921. doi: 10.1128/mbio.03239-21. Epub ahead of print.
Other associated proteins
|PF3D7_0720700||phosphoinositide-binding protein px1|