Live-cell imaging of microtubule and centriolar plaque reorganization throughout schizogony. (A) Schematic of P. falciparum blood-stage development including multiple divisions (schizogony) before cytokinesis and egress of new infectious parasites. (B) Deconvolved confocal live-cell still images of two separate Plasmodium NF54 infected red blood cells ectopically expressing PfCentrin1-GFP (green), labeled with SPY555-Tubulin (magenta) and 5-Sir-Hoechst (blue). The images are maximum intensity projections. (C) Time-lapse of a cell labeled as in (B), but without 5-Sir-Hoechst. The first spindle formation and elongation in a single parasite is shown over time.
Simon CS, Funaya C, Bauer J, Voβ Y, Machado M, Penning A, Klaschka D, Cyrklaff M, Kim J, Ganter M, Guizetti J. An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites. Life Sci Alliance. 2021 Sep 17;4(11):e202101199.
Other associated proteins