PF3D7_0422300 alpha tubulin 2, transcript expression profile microgametocyte

Centriolar plaques are divided in an extranuclear centrin-containing compartment and an intranuclear DNA-free and protein-dense compartment associated with microtubules(E) Correlative in-resin widefield fluorescence and electron tomography (CLEM) images of thick sections (300 nm) of a high-pressure frozen and embedded NF54 schizont expressing PfCentrin1-GFP (green) and stained for DNA with 5-SiR-Hoechst (blue). Same cell region containing clear PfCentrin1-GFP foci imaged by fluorescence microscopy was overlayed with an electron tomogram slice. In zoom-ins, arrows indicate a microtubule (magenta) and a boundary-like region (black) for two tomogram slices. (F) Confocal images of individual 3D7 Nup313-3xHA_glms schizont nuclei expanded with U-ExM in hemispindle and mitotic spindle phase. Proteins were labeled in bulk using an NHS-ester Atto594 dye conjugate (white). Brighter staining therefore indicates higher protein density. Cells were additionally stained with anti-tubulin (magenta) and Hoechst (blue).suggests a boundary-like

Simon CS, Funaya C, Bauer J, Voβ Y, Machado M, Penning A, Klaschka D, Cyrklaff M, Kim J, Ganter M, Guizetti J. An extended DNA-free intranuclear compartment organizes centrosome microtubules in malaria parasites. Life Sci Alliance. 2021 Sep 17;4(11):e202101199.

Other associated proteins

PFID Formal Annotation
PF3D7_0107000 centrin-1
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