BODIPY–ceramide staining of GFP transfectant lines. Dual labelling of SBPGFP (A), PFE55END (B) or PFA660END (C) with BODIPY–ceramide. Fluorescence channels are shown individually in black/white for highest contrast. All images are maximal projections of Z-stack serial sections. In merge image green, GFP; red, BODIPY. Arrows in (A) show regions in which GFP and BODIPY signals colocalize, arrowheads regions in which no colocalization can be visualized. In infected erythrocytes containing Maurer’s clefts targeted PfSBP1–GFP, many, but not all, of the GFP-labelled structures were also positive for the membrane stain (A, compare white arrows). In stark contrast, in erythrocytes infected with PFE55END, PFA660END GFP signals did not colocalize with regions of membrane staining (B and C).
Külzer S, Rug M, Brinkmann K, Cannon P, Cowman A, Lingelbach K, Blatch GL, Maier AG, Przyborski JM. Parasite-encoded Hsp40 proteins define novel mobile structures in the cytosol of the P. falciparum-infected erythrocyte. Cell Microbiol. 2010 12(10):1398-420.
Other associated proteins
|PF3D7_0113700||heat shock protein 40, type II|
|PF3D7_0501100||co-chaperone j domain protein jdp|