Generation and validation of HSP101-HAglmS parasite line and knockdown analysis. Immunofluorescence analysis of ring-stage parasites treated with 2.5mMGlcN for 48 h and fixed with acetone/methanol and labelled with anti-HA antibodies to detect HSP101-HA, or antibodies against exported proteins SBP1 and GEXP07. This demonstrates that knocking down expression of HSP101-HA blocks the export of SBP1 and GEXP07 into the host erythrocyte. Parasite nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole). Scale bar, 5 μm.

Gabriela M, Matthews KM, Boshoven C, Kouskousis B, Jonsdottir TK, Bullen HE, Modak J, Steer DL, Sleebs BE, Crabb BS, Koning-Ward TF, Gilson PR. A revised mechanism for how Plasmodium falciparum recruits and exports proteins into its erythrocytic host cell. PLoS Pathog. 2022 18(2):e1009977.