Generation of the NF54 Rhoph2/EXP2 line. Live fluorescence microscopy images indicating the expected localization of the tagged proteins. Red arrows indicate the dual localization of RhopH2-mNG on the RBC membrane as well as the parasite in the rings and trophozoites, while in the schizonts, the protein is localized to rhoptries. The EXP2-mRuby marks the PVM of the parasite. Erythrocyte membrane is stained with WGA-Alexa 350 (blue).

Ahiya AI, Bhatnagar S, Morrisey JM, Beck JR, Vaidya AB. Dramatic Consequences of Reducing Erythrocyte Membrane Cholesterol on Plasmodium falciparum. Microbiol Spectr. 2022; 10(1 ):e0015822.