(C) Brightfield (BF), fluorescence images (top) of live parasites episomally expressing PPS-GFP and stained with 10 nM Mitotracker Red and (bottom) immunofluorescence analysis (IFA) images of fixed parasites episomally expressing PPS-RFP stained with anti-RFP and anti-apicoplast ACP antibodies. (D) IFA images of fixed parasites expressing PPS-RFP that had been treated for >7 days with 2 μM doxycycline (Dox) and 200 μM IPP (to stably induce apicoplast loss) and stained with anti-RFP antibody to visualize PPS distribution

Okada M, Rajaram K, Swift RP, Mixon A, Maschek JA, Prigge ST, Sigala PA. Critical role for isoprenoids in apicoplast biogenesis by malaria parasites. Elife. 2022 11:e73208. PMID: 35257658