PF3D7_0208500 acyl carrier protein

Inhibition of isoprenoid precursor biosynthesis specifically blocks apicoplast elongation and branching. NF54 parasite line (PfMev) that expresses ACPL-GFP (acyl carrier protein (ACP) leader sequence fused to GFP) as well as heterologous enzymes that enable cytoplasmic synthesis of IPP from exogenous mevalonate precursor, independent of the apicoplast MEP pathway. Bright field (BF) and fluorescent microscopy images of live NF54 PfMev parasites that were (A) untreated or treated with 10 μM fosmidomycin (FOS) in the absence or presence of 50 μM DL-mevalonate (Mev), or (B) treated with 6 μM blasticidin-S (177 Blast-S), 2 μM DSM1, 100 nM atovaquone (ATV), or 5 nM WR99210. (D) Live-cell imaging of ΔDXS PfMev parasites in the presence or absence of 50 μM Mev.

Okada M, Rajaram K, Swift RP, Mixon A, Maschek JA, Prigge ST, Sigala PA. Critical role for isoprenoids in apicoplast biogenesis by malaria parasites. Elife. 2022 11:e73208. PMID: 35257658.

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