AIRYSCAN CONFOCAL FLUORESCENCE IMAGING MICROSCOPY OF MNEONGREEN-TAGGED PFRACK1EXPRESSED IN P. FALCIPARUM. (A) Hoechst Stain (DNA), BODIPY TR ceramide (lipid bilayers), mNeonGreen (RACK1), Transmitted-photomultiplier tube (T-PMT) as brightfield, and overlay images. Top) Dd2 parent line. Middle) Dd2 parent line expressing mNeonGreen-tagged PfRACK1 at trophozoite stage. Bottom) Dd2 parent line expressing mNeonGreen-tagged PfRACK1 at schizont stage. (B) Two-dimensional overlay using Imaris software analysis of Airyscan images. Left) Dd2 parent line at the schizont stage. Right) Dd2 parent line expressing mNeonGreen-tagged PfRACK1 in trophozoite and schizont stages. The size bar in the images represents 5 μM.

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