Organelle labelling and immunostaining of PfPSS-GFP parasites establish that the
PfPSS is an ER-resident protein. (A) Fluorescence microscopy images of PfPSS-GFP. B Schizont Trophozoite Ring
A PfPSS-GFP αBip Merged Merged+DIC
Figure 2 parasites at different developmental stages, imuno-stained with antibodies against ER marker protein BiP. The PfPSS-GFP was found to be colocalized with the BiP staining at all the stages of the parasite asexual life cycle. (B) Fluorescence confocal microscopy images of PfPSS-GFP transgenic line: (i) immuno-stained with antibody against apicoplast resident protein PfClpP; (ii) immuno-stained with antibody against Cis-Golgi marker protein PfGRASP; and (iii) labelled with live fluorescent mitochondria dye MitoTracker Red. Parasite nuclei were stained with DAPI. The PfPSS-GFP localization was found to be distinct from all the three labelled organelles of parasite.
Anwar O, Islam M, Thakur V, Kaur I, Mohmmed A. Defining ER-mitochondria
contact dynamics in Plasmodium falciparum by targeting component of phospholipid synthesis pathway, Phosphatidylserine synthase (PfPSS). Mitochondrion. 2022 :S1567-7249(22)00046-0. PMID: 35623558.
Other associated proteins
|PF3D7_0307400||ATP-dependent Clp protease proteolytic subunit|
|PF3D7_1017300||golgi re-assembly stacking protein 2|
|PF3D7_1366800||phosphatidylserine synthase, putative|