Indirect immunofluorescence assays (IFAs) of indicated proteins in wild-type (WT) and transfected lines. In schizonts (top), CLAG3 colocalizes with RhopH3 in apical rhoptries (puncta) in 8-1HA but has a more diffuse distribution in 8-1HAtrunc. IFA with trophozoite-stage parasites (bottom) reveals a normal export of CLAG3 and colocalization with RhopH3 at the host membrane in 8-1HA but a reduced signal with failed export in 8-1HAtrunc. Scale bars, 5 mm. Imaging revealed that the tandem-tagged CLAG3 protein in 8-1HA trafficked as expected and colocalized with RhopH3 at the host cell surface

Brzostowski JA, Desai SA. Kinetic Tracking of Plasmodium falciparum Antigens on Infected Erythrocytes with a Novel Reporter of Protein Insertion and Surface Exposure. mBio. 2022 Jun 28;13(3):e0040422.