Localization of PfSAMS-HA in the asexual blood stages and gametocytes. Immunofluorescence assays were employed, using rat anti-HA antibody, to detect PfSAMS-HA in trophozoites (TZ), schizonts (SZ), and gametocytes (GC) of stages II–V (green), of the PfSAMS-HA-KD parasite line. Counterlabeling of the asexual blood stages was done using mouse anti-Pf 92 antisera and of gametocytes with rabbit anti-Pf s230 antisera (red). The nuclei were labeled with Hoechst 33342 nuclear stain (blue). Bar; 5 mm.
Musabyimana JP, Distler U, Sassmannshausen J, Berks C, Manti J, Bennink S, Blaschke L, Burda PC, Flammersfeld A, Tenzer S, Ngwa CJ, Pradel G. Plasmodium falciparum S-Adenosylmethionine Synthetase Is Essential for Parasite Survival through a Complex Interaction Network with Cytoplasmic and Nuclear Proteins. Microorganisms. 2022 Jul 14;10(7):1419. doi: 10.3390/microorganisms10071419. PMID: 35889137
Other associated proteins
|PF3D7_0209000||6-cysteine protein p230|
|PF3D7_1364100||6-cysteine protein p92|