Dissection of the dual localization of P. falciparum TrxR. (A) Dual localization (cytosol and mitochondria) of TrxR-GFP effected by a newly discovered N-terminal extension/leader. (B) Mitochondrial targeting of a construct (TrxR-N76-GFP) containing solely the TrxR 59-extension. (C) Detection of TrxR-GFP fusion constructs in transgenic parasites by Western blot analysis against the GFP-moiety, showing that the dual localization of TrxR-GFP is not due to a weak mitochondrial signal sequence.
Kehr S, Sturm N, Rahlfs S, Przyborski JM, Becker K. Compartmentation of redox metabolism in malaria parasites. PLoS Pathog. 2010 6(12):e1001242.