Trafficking and membrane insertion for large multi-HiBiT insertions into the CLAG3 HVR. IFA micrographs of indicated lines at the schizont and trophozoite stages (A and B, respectively), showing that each construct is trafficked faithfully to rhoptry organelles in schizonts but that the multi-HiBiT lines do not export CLAG3 to the host membrane of most trophozoite-infected cells. Scale bars, 5 μm.

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